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 7th July 2008 GE Healthcare

GE Healthcare introduces trial kit for 2-D DIGE experiments

GE Healthcare has introduced a new protein labeling trial kit for 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE), offering a quick, easy and low-cost opportunity for scientists new to 2-D DIGE to test the system. The DIGE Trial Pack contains 2 nmol each of the three CyDye™ DIGE Fluor minimal dyes Cy™2, Cy3 and Cy5, sufficient to run 5 gels (10 protein samples plus internal standards), and a free one month trial license of DeCyder™ 2-D Differential Analysis Software, the dedicated image analysis software for the Ettan DIGE system.

The 2-D DIGE system offers significant cost and time-saving benefits in comparison with classical 2-D electrophoresis. Protein mixtures are labeled prior to electrophoresis with size and charge-matched, spectrally resolvable CyDye fluors, allowing the simultaneous separation and comparative analysis of up to three samples on a single 2-D gel. Following 2-D electrophoresis, the results may be scanned with the Typhoon™ Variable Mode Imager or Ettan™ DIGE Imager. DeCyder Differential Analysis Software automatically locates and analyzes protein spots, assigning statistical confidence to every difference between the samples. As the system ensures that each protein spot has its own internal standard, gel variation issues are eliminated, significantly increasing accuracy and reproducibility.

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