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 30th September 2009 Promega

NEW Promega GoTaq® qPCR Master Mix Provides Greater

Sensitivity and Stability

Southampton, UK. (24 September, 2009) The new Promega GoTaq® qPCR Master Mix, provides unprecedented PCR sensitivity, enabling the detection of smaller changes and rare events. In addition, the new GoTaq® qPCR Master Mix delivers earlier cycle thresholds than SYBR® Green I.

The sensitivity of GoTaq® qPCR Master Mix is derived from a new fluorescent dye that gives a brighter signal when it binds to double-stranded DNA, compared to the SYBR® Green I, and is more resistant to inhibitors.

Derek McCall, Managing Director of Promega UK, said, “The advanced functionality provided by our qPCR Master Mix gives scientists both increased reliability and reproducibility compared to the performance of existing real-time reagent systems. And that means high performance even with low expression targets.”

Stability Facilitates Automation

GoTaq® qPCR Master Mix offers enhanced stability at ambient and elevated temperatures, making it ideal for automated applications. Also, GoTaq® qPCR Master Mix remains stable after 24 hours at ambient temperature. Even after incubating GoTaq® at extreme temperatures (96°C) for 90 minutes, Cq values remain unchanged. In contrast, other leading qPCR Master Mixes fail to amplify after 30 minutes of exposure to elevated temperatures.

Replaces SYBR

The set up for GoTaq® is similar to SYBR® Green l, using the same cycling conditions and detection channel. GoTaq® qPCR Master Mix contains a low level of reference dye (CXR) that can be detected using the same filters and setting as those for ROX™. In addition, GoTaq® qPCR Master Mix can be used with any real-time instrument capable of detecting SYBR® Green I or FAM™ dye.

UK scientists are invited to take the GoTaq qPCR Challenge. By submitting data comparing their current real-time PCR reagents to GoTaq®, five scientists per month* can qualify for 50% off the GoTaq® qPCR Master Mix for a year.

* Visit gotaqchallenge.com for terms and conditions


 

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